Journal of Medicinal Materials, 2023, Vol. 28, No. 4 (pp. 199 - 205)

ISOLATION OF TRITERPENES FROM DISDICHIA MAJOR

AND ESTABLISHMENT OF β-AMYRIN AND LUPEOL

AS REFERNCE SUBSTANCES

Tran Thi Van Anh¹, Tran Trung Trinh^{1, 2}, Phan Van Ho Nam^1,*

¹Faculty of Pharmacy, University of Medicine and Pharmacy at HoChiMinh City, Vietnam;

²Faculty of Pharmacy, HongBang University, Vietnam

*Corresponding author: phanvanhonam@ump.edu.vn

(Received March 10^th, 2023)

Summary

Isolation of Triterpenes from Dischidia major and Establishment of β-Amyrin and Lupeol as Reference Substances

In this study, we reported the isolation of three main triterpenes from alcohol extract of Dischidia major (DM) including β-amyrin, α-amyrin and lupeol. The structures of isolated compounds were identified by UV, IR, NMR and MS spectrometry. β-Amyrin and lupeol were used for establishing reference compounds according to ISO GUIDE 35:2017 and ISO 13528:2022. The purity of β-amyrin and lupeol was determined as 99.81% and 99.80% on the basis of dry weight by HPLC-PDA, respectively.

Keywords: Dischidia major, α-Amyrin, β-Amyrin, Lupeol, Reference substance.

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