Journal of Medicinal Materials, 2023, Vol. 28, No. 4 (pp. 199 - 205)
ISOLATION OF TRITERPENES FROM DISDICHIA MAJOR
AND ESTABLISHMENT OF β-AMYRIN AND LUPEOL
AS REFERNCE SUBSTANCES
Tran Thi Van Anh1, Tran Trung Trinh1, 2, Phan Van Ho Nam1,*
1Faculty of Pharmacy, University of Medicine and Pharmacy at HoChiMinh City, Vietnam;
2Faculty of Pharmacy, HongBang University, Vietnam
*Corresponding author: firstname.lastname@example.org
(Received March 10th, 2023)
Isolation of Triterpenes from Dischidia major and Establishment of β-Amyrin and Lupeol as Reference Substances
In this study, we reported the isolation of three main triterpenes from alcohol extract of Dischidia major (DM) including β-amyrin, α-amyrin and lupeol. The structures of isolated compounds were identified by UV, IR, NMR and MS spectrometry. β-Amyrin and lupeol were used for establishing reference compounds according to ISO GUIDE 35:2017 and ISO 13528:2022. The purity of β-amyrin and lupeol was determined as 99.81% and 99.80% on the basis of dry weight by HPLC-PDA, respectively.
Keywords: Dischidia major, α-Amyrin, β-Amyrin, Lupeol, Reference substance.
(Nguồn tin: )