Journal of Medicinal Materials, 2020, Vol. 25, No. 3 (pp. 186 - 192)
PUTATIVE CONSTITUENTS CONTRIBUTING TO THE ANTIDEPRESSANT-LIKE EFFECTS OF OCIMUM SANCTUM
IN OLFACTORY BULBECTOMIZED-MICE
Nguyen Thu Hien1, Le Thi Xoan1,*, Phung Nhu Hoa2, Nguyen Van Tai2, Nguyen Minh Khoi2
1Department of Pharmacology and Biochemistry, National Institute of Medicinal Materials, Hanoi, Vietnam;
2Department of Phytochemistry, National Institute of Medicinal Materials, Hanoi, Vietnam
*Corresponding author: xoanle@nimm.org.vn
(Received May 18th, 2020)
Summary
Putative Constituents Contributing to the Antidepressant-Like Effects of Ocimum sanctum
in Olfactory Bulbectomized-Mice
The ethanolic extract of Ocimum sanctum Linn. (OS) improves depression-like behaviors in olfactory bulbectomized (OBX) mice, an animal model of depression, had previously reported. This study aimed to clarify the putative constituents contributing to the anti-depressant effects of the OS extract on OBX mice. OBX mice were daily treated with an ethanolic extract of OS or its fractions including the n-hexane-, ethyl acetate (EtOAc), and n-butanol (BuOH) (400 mg/kg, p.o) or a reference drug, imipramine (IMP) (8.0 mg/kg, i.p.), one week before and continuously for three days after surgery. The anti-depressant effect was measured using a tail suspension test (TST) and forced- swimming test (FST). Chemical structures of the isolated compounds from the OS fraction which showed the significance anti-depression effect was determined using spectroscopic methods. In TST, treatment of OS extract and BuOH fraction but not n-hexane and EtOAc fractions significantly shortened the immobility time of OBX mice as IMP treatment did. The administration of OS extract, BuOH fraction, and imipramine but not n-hexane and EtOAc fractions significantly increased the climbing time of OBX animals in the FST. The present results demonstrated that OS extract ameliorated OBX-induced depression-like behavior in mice and that the components in BuOH fraction including apigenin (1), luteolin (2), and apigenin-7-O-β-D-glucuronide (3) may play a role on the action of OS extract.
Keywords: Anti-depressant, Ocimum sanctum, Olfactory bulbectomized mice, Constituents.
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