Ấn phẩm

Quantitative Determination of Daphnoretin in Aerial Parts of Wikstroemia indica L. (Thymelaeaceae) by HPLC-DAD(Journal of Medicinal Materials, 2023, Vol. 28, No. 2)

Journal of Medicinal Materials, 2023, Vol. 28, No. 2 (pp. 93 - 98)

 

QUANTITATIVE DETERMINATION OF DAPHNORETIN IN AERIAL PARTS OF WIKSTROEMIA INDICA L. (THYMELAEACEAE) BY HPLC-DAD

Pham Thi Hien1, Nguyen Thi Ha Ly1, Nguyen Van Lieu1, Vu Thi Diep1, Ho Hoai Thuong4,

Nguyen Cong Luong3, Do Thi Ha1,*

1National Institute of Medicinal Materials, Hanoi, Vietnam; 2Vietnam Military Medical University;

3Traditional Medicine Administration, Ministry of Health, Vietnam;

4University of Science, Vietnam National University, Hanoi, Vietnam

*Corresponding author: hado.nimms@gmail.com

(Received March 26th, 2023)

Summary

Quantitative Determination of Daphnoretin in Aerial Parts of Wikstroemia indica L. (Thymelaeaceae) by HPLC-DAD

A simple and sensitive HPLC method was proposed for the quantification of daphnoretin in aerial parts of Wikstroemia indica (L.) (W. indica). The chromatographic separations were obtained by an Agilent C18 column (250 x 4.6 mm, 5 µm particle size) using isocratic elution with acetonitrile and water (40/60, v/v), at a flow rate of 0.6 mL/min, with an injection volume of 10 µL and detection wavelength of 345 nm. The calibration curve displayed a good linear relationship (linearity ranging from 14.88 - 238 µg/mL and R2=0.9999). The method was validated for system suitability (RSD of peak area = 0.21%; RSD of tR = 0.15%); precision (RSD < 3.0%) and accuracy (recovery: 95.60-102.92%). The validation results displayed good accuracy, repeatability, linearity, and selectivity according to the ICH guideline. The percentage content of daphnoretin in aerial part samples of W. indica ranged from 0.010% to 0.207%. The obtained results provided useful information for quality assurance and standardization of W. indica in Vietnam.

Keywords: Wikstroemia indica (L.), Aerial parts, HPLC-DAD, Daphnoretin.

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