Journal of Medicinal Materials, 2020, Vol. 25, No. 3 (pp. 150 - 156)
STUDY ON ISOLATION AND STANDARD ESTABLISHMENT OF SINENSETIN FROM EXTRACTUM HERBA ORTHOSIPHONIS SPIRALIS
Huynh Tran Quoc Dung1, Phan Thi Thuy Tien2, Pham Ngoc Thac1, Pham Thai Trong Nhan3, Nguyen Duc Hanh3,*, Nguyen Minh Duc3,4
1Traditional Medicine Hospital, Ho Chi Minh City, Vietnam; 2OPC Pharmaceutical Joint Stock Company, Vietnam
3Faculty of Pharmacy, University of Medicine and Pharmacy at Ho Chi Minh City, Vietnam;
4Faculty of Pharmacy, Ton Duc Thang University, Vietnam
*Corresponding author: firstname.lastname@example.org
(Received May 20th, 2020)
Study on Isolation and Standard Establishment of Sinensetin
from Extractum Herba Orthosiphonis spiralis
Orthosiphon spiralis, a well-known herbal medicine, is used for clearing Damp-Heat in the kidneys, bladder, promoting urination, and reducing kidney stones to treat high blood pressure and relieving pain in rheumatoid arthritis. Sinensetin is an active compound of O. spiralis, used for quality control of O. spiralis. The study aimed to isolate sinensetin from Extractum Herba Orthosiphonis spiralis using different chromatography techniques such as thin-layer chromatography, column chromatography, and preparative HPLC. The isolated sinensetin was demonstrated to meet the requirement of a reference standard based on ISO Guide 34 and 35. From 2.2 kg of Extractum Herba Orthosiphonis spiralis, 1.17 g of the isolated sinensetin was obtained with the sinensetin content of 99.83%.
Keywords: Extractum Herba Orthosiphonis spiralis, Sinensetin, Reference standard.
(Nguồn tin: )