Journal of Medicinal Materials, 2017, Vol. 22, No. 3 (pp.162-166)

IN VITRO ACETYLCHOLINESTERASE INHIBITION AND FREE RADICAL SCAVENGING ACTIVITY OF ACANTHOPANAX GRACILISTYLUS W.W. SMITH EXTRACT

OLAPOJU Samuel O.¹, Pham Thi Nguyet Hang^2,*

1. Vietnam-France University; ²National Institute of Medicinal Materials, Hanoi, Vietnam

*Corresponding author: pnhang2004@yahoo.com

(Received April, 07^th, 2017)

Summary

In vitro Acetylcholinesterase Inhibition and Free Radical Scavenging Activity of Acanthopanax gracilistylus W.W. Smith Extract

This research was designed to evaluate the in vitro acetylcholinesterase (AChE) inhibitory ability and antioxidant effect of Acanthopanax gracilistilylus W.W. Smith (AG) ethanol extract. The effect of AG extract on AChE was evaluated by Ellman method and antioxidant effect of AG was investigated by using DPPH and superoxide scavenging in vitro assay. The results from the AChE inhibition showed that AG extract has the ability to inhibit AChE up to 79.80% at concentration 3.0 mg/ml with an IC₅₀ value of 1.50 mg/ml.  The in vitro DPPH free radical scavenging activity of AG extract was found to be up to 88.67% at concentration 250 µg/ml with an IC₅₀ value of 88.90 µg/ml. AG extract also showed a weak superoxide scavenging effect and at 3.00 mg/ml, 57.59% of superoxide was inhibited with IC₅₀ found to be 2.70 mg/ml.

Keywords: Acanthopanax gracilistilylus ethanol extract, Acetylcholinesterase inhibition, Radical scavenging, DPPH, Superoxide.

(Nguồn tin: Tạp chí Dược liệu - Viện Dược liệu)