Determination of Some Active Components in Herba Orthosiphoris spiralis by TLC-Scanning for Standardization
Thin layer chromatography- fingerprint (TLC-FP) identification of flavonoids, terpennoids and caffeic acid derivatives together with the quantitative dethermination of sinensetin and ursolic acid on Herba Orthosiphonis spiralis were performed. Solvent systems of dichloromethan : ethylacetat 6: 4 (1), chloroform : ethylacetat 5 : 2 (2) and toluen : ethylacetat: aceton : acid formic 5: 2: 2: 1 (3) were used for separation of the group: flavonoids, terpenoids and sinensetin, respectively. Detection of the flavonoids and caffeic acid derivatives are observed under UV light at 366nm before and after spraying with NP/ PEG reagent. For detection of terpennoids, visualization by spraying with sulfuric acid 10% and heating. For quantitative analysis of sinensetin and ursolic acid , the solvent system (1) was applied. Fluorometric scanning at l366nm , K400 for quantitation of sinensetin and scanning at l 520 nm for quantitation of ursolic acid after spraying with10% sulfuric acid solution in ethanol and heat at 110 0 C for 5 minute. The calibration curves linear at 0,05- 0,25 mg (sinensetin) and 0,5- 2,5 mg (ursolic acid), precision (RSD) 2,25 % and 4,88 % (n =6), average recovery 100,95% and 100,01%, respectively. The content of sinensetin and ursolic acid in the herba are estimated 0,017 - 0,044% and 0,20 - 0,39% (n = 6), respectively.
Key words: Fluorometric scanning, qualitative, quantitative, sinensetin, ursolic acid, caffeic acid derivatives
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