Journal of Medicinal Materials, 2017, Vol. 22, No. 3 (pp.131-136)
ENNIATIN DERIVATIVES FROM FUSARIUM SP. AND THEIR BIOLOGICAL ACTIVITIES
Hoang Duc Manh1, Nguyen Minh Khoi1, Tran Minh Ngoc1, Do Thi Ha1,
Bo Yeon Kim2, Jong Seog Ahn2, Tran Manh Hung3,*
1National Institute of Medicinal Materials, Hanoi, Vietnam;
2Korea Research Institute of Bioscience and Biotechnology (KRIBB), 685-1 Yangcheonri, Ochangup, Cheongwongun 363-883, Korea;
3Biomedical Science Department, VNUK-Institute for Research & Executive Education, The University of Danang, Danang City, Vietnam
*Corresponding author: firstname.lastname@example.org
(Received February, 06th, 2017)
Enniatin Derivatives from Fusarium sp. and their Biological Activities
The genus Fusarium sp. has been reported to contain several phytochemical constituents with applicable pharmaceutical properties. In our experiment, bioassay-guided fractionation resulted in the isolation of eight compounds from the ethyl acetate-soluble extract of Fusarium sp. FN332 fungi broth. Their chemical structures were elucidated as enniatin B (1), enniatin H (2), enniatin A (3), enniatin F (4), enniatin I (5), MK 1688 (6), enniatin D (7), and enniatin G (8) on the basis of spectroscopic data. All the isolated compounds (1−8) inhibited PTP1B with IC50 values ranging from 12.7 ± 0.5 to 35.5 ± 1.2 μM. Enniatin A (3) reduced the Vmax value, but did not alter the Km value of PTP1B in kinetic action. Enniatin A (3) also stimulated the phosphorylation of AMPKα catalytic subunit in a dose-dependent manner. The results suggest that the enniatins from Fusarium sp. might be a considerable source of PTP1B inhibitor and AMPK phosphorylation activator.
Keywords: Fusarium sp, Enniatin, AMPKα catalytic, PTP1B inhibitor.
(Nguồn tin: Tạp chí Dược liệu - Viện Dược liệu)