Study on in vitro Cytotoxic Activity of Ganoderma lucidum, Ganoderma colossum, Trametes versicolor, Phellinus linteus on Three Cancer Cell Lines
Ganoderma lucidum, Trametes versicolor (also called Coriolus versicolor), Phellinus linteus are favorite remedies in Oriental medicine. For centuries, they have been used for prevention and treatment of many human diseases. Studies on these mushrooms have demonstrated their anti-tumor and immunomodulatory activities. Ganoderma colossum is a Vietnamese mushroom. Up to date, they are no many studies about this mushroom. This study aimed to evaluate the in vitro cytotoxic activity of ethanol extracts and water extracts prepared from G.lucidum, G.colossum, T.versicolor and P.linteus on three cancer cell lines (Hela cervical cancer, MCF-7 breast cancer and NCI-H460 lung cancer cell line using sulforhodamine B assay. At the concentration of 100 μg/ml, the ethanol extract of G. colossum showed the highest cytotoxic activity on three cancer cell lines compared with other extracts, and the Hela cervical cancer cell line tended to be more sensitive compared with other two cell lines. The antiproliferation effect of the G. colossum ethanol extract on Hela cancer cell line was expressed with IC50= 49.64 ± 9.06 μg/ml. This study indicates that ethanol extract of G.colossum has the anti-tumor activity, inhibits the proliferation of Hela cervical cancer cell line in vitroand suggests further studies on investigating its mechanism(s) of action.
Key words: Ganoderma lucidum, Ganoderma colossum, Trametes versicolor, Phellinus linteus, Sulforhodamine B assay, Hela cervical cancer cell line
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