Hairy-Roots Cultures of Artemisia annua L. for Artemisinin

Accumulation by Immersion Bioreactor Technique

The improved MS media was set up with 26 ± 2 ^oC of temperate room, 16 hours of fluorescent lighting and pH = 6 enhanced the artemisinin accumulation up to 1.982%; combined with immersion bioreactor set up to sink/floating (15/45 minutes) increased artemisinin accumulation up to 2.088%. Yeast extract or chitosan used as elicitors stimulate significantly the artemisinin accumulation. Hairy-roots cultures of Artemisia annua L. for artemisinin production by bioreactor techniques were established.

Keywords: Artemisia annua L.; immersion bioreactor; sink/floating; accumulation; hairy-roots; elicitors.

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