Journal of Medicinal Materials, 2023, Vol. 28, No. 3 (pp. 166 -  172)

OPTIMIZATION OF THE EXTRACTION OF TOTAL FLAVONOIDS

FROM LAC TIEN (PASSIFLORA FOETIDA L.)

USING THE RESPONSE SURFACE METHODOLOGY

Le Xuan Duy¹, Do Thi Thuy Linh¹, Nguyen Phu Quang¹, Nguyen Thi Kim Anh¹,

Phung Van Trung², Nguyen Minh Khoi¹, Nguyen Tuan Hiep^1,*

¹National Institute of Medicinal Materials (NIMM), Hanoi, Vietnam;

²Center for Research and Technology Transfer, Vietnam Academy of Science and Technology (VAST), Hanoi, Vietnam

*Corresponding author: nguyentuanhiep.2710@hotmail.com

(Received March 29^th, 2023)

Summary

Optimization of the Extraction of Total Flavonoids from Lac Tien (Passiflora foetida L.)
Using the Response Surface Methodology

This study aims to optimize the extraction conditions of total flavonoids from Passiflora foetida L. using the reflux extraction method. The experimental layout is carried out according to the Box - Benhken design method. Using response surface methodology (RSM), an optimal model of the extraction process has been built with two objective functions: extraction efficiency (Y1) and total flavonoid content (Y2); four factors of extraction, which are ethanol concentration, extraction temperature, extraction time and extraction ratio, are studied. Based upon RSM data, the optimal conditions are as follows: ethanol concentration 71.7 (%, v/v), extraction temperature 68.09^oC, extraction time 151.18 (minutes), and extraction ratio 25.2/1 (mL/g). At this condition, the extraction efficiency and total flavonoid content in the extract reach the maximum value of 14.95 ± 0.16% and 2.39 ± 0.09%, respectively.

Keywords: Response Surface Methodology (RSM), Passiflora foetida L., Total flavonoid content.

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