CYTOTOXIC ACTIVITY OF ABIETANE-TYPE DITERPENES FROM SALVIA MILTIORRHIZA BUNGE CULTIVATED IN HA GIANG PROVINCE

Pham Thanh Binh¹, Bui Thi Mai Anh¹, Nguyen Hai Dang², Pham Van Cuong^1,2, Nguyen Van Hung¹, Pham Thi Thu Hanh¹, Nguyen The Cuong³, Luong Trieu Vung⁴, Giang Loc Thang⁵, Nguyen Tien Dat^1,*

¹Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST),

Hanoi, Vietnam;

²Advanced center for Bio-organic Chemistry, IMBC, VAST;

³Institute of Ecology and Biological Resources, VAST;

⁴Department of Agriculture and Rural Development of Ha Giang;

⁵Pho Bang Centre for Plant and Animal Varieties, Dong Van, Ha Giang

*Corresponding author: ngtiend@imbc.vast.vn

(Received September, 04^th, 2016)

Summary

Cytotoxic Activity of Abietane-type Diterpenes from Salvia miltiorrhiza Bunge Cultivated in Ha Giang Province

A phytochemical fractionation of the methanol extract of the roots of Salvia miltiorrhiza cultivated in Ha Giang province led to the isolation of four abietane-type diterpenes, which were identified as 6,7-didehydroferruginol (1), ferruginol (2), tanshinone IIA (3), and sibiriquinone B (4). Compound 1 was identified for the first time from S. miltiorrhiza. All compounds were evaluated for their cytotoxicity against human lung carcinoma (A549, EGFR-TKI resistant, wild type), human breast carcinoma (MDA-MB-231), and human prostate carcinoma (DU145) cell lines. Compound 1 showed potent cytotoxic effect against EGFR-TKI resistant A549 cells with the IC₅₀ value of 0.68 μM.

Keywords: Salvia miltiorrhiza, Abietane-type diterpene, 6,7-didehydroferruginol, EGFR-TKI resistant.