Summary

Cell Cloning of Taxus sp. for Taxol Accumulation

In vitro plantlets of Taxus sp. were used as planting materials. The favoured medium initiates and proliferates of callus was WPM + 2.4 D (5 - 4 mg/l). The favoured medium initiates callus cell suspension was B5 + 2.4 D (3 mg/l) and proliferates of cell suspension was B5 + 2.4 D (3 mg/l) + NAA (3 mg/l). Time period for subculture of cell suspension was 42 days after being cultured. Cell cloning was done through 3 years based on fast cell growth and development and selection to 2 clones from sources of stems and leaves having taxol accumulation separately TĐL1 = 0.03826 % taxol, TĐT1 = 0.02855 % taxol. We have already established the primary way to extract and quantify taxol by HPLC using solvent MeO

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