Studies on the Effects of Elicitors on Taxol Accumulation in Taxus wallichiana Zucc. Cell Culture
Media for taxus cell propagation was determined on MS + 3 mg/l 2.4D + 3 mg/l NAA + 0.5 mg/l kinetin + 0.1 mg/l BA + 10% CW. Cell selections were carried out through 8 intervals in the year of 2010 with interval cultivation time of 45 days/each step. It could improve the taxol accumulation via cell suspension cultures sourced from leaves 170.1 mg/gDW and stems 27.3 mg/gDW. Picloram was not effect on taxol accumulation.
On the basic media MS + 3 mg/l 2.4D + 3 mg/l NAA + 0.5 mg/l kinetin + 0.1 mg/l BA + 10% CW supplemented with precursor of 15mg/l phenyl alanine (PA) effects on the percentage of FW/DW (fresh weight/dried weight) was 9.815 and the taxol accumulation was 0.778%. The effects of elicitors supplemented to media with 10 mg/l methyl jasmonate (MJ) having 7.183 FW/DW and 0.273% taxol, 100 mg/l salysilic acid (SA) having 10.12 FW/DW and 0.094% taxol, 50 mg/l chitosan having 10.09 FW/DW and 0.119% taxol, 5 mg/l oligo-chitosan having 9.090 FW/DW and 0.778% taxol. The effects of the combination of precursor and elicitors (15 mg/l phenyl alanine, 10 mg/l methyl jasmonate, 5 mg/l oligo-chitosan, 100 mg/l salysilic acid) enhance the taxol accumulation to 1.003% in comparison of separately as PA having 0.114% taxol, PA+Ochi having 0.542% taxol, PA+MJ+Ochi having 0.564%. Cell cloning from leaves (1.701%) had the taxol accumulation more than from stem (0.273%)
Keyword: Cell propagation, cell selection, cell suspension, precursor, elicitor.
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