Studies the Possibility of Production of Lovastatin were Selected by
Engineering Mutant from Aspergillus terreus Strains
Lovastatin is a potent cholesterol-lowering drug. Lovastatin acts by competitively inhibiting the enzyme 3- hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA) which catalyses the rate-limiting step of cholesterol biosynthesis. Lovastatin is produced as a secondary metabolite by a variety of filamentous fungi: including Penicillum sp., Monascus rubber and A. terreus. Random mutation and selection strategies have been reported for obtaining overproducing isolates of A. terreus .
In the present study, we have studied the possibility of production of lovastatin A. terreusstrains selected by engineering mutant. A mutation-selection program was carried out with A. terreus ATCC 20542 and A. terreus VTCC-F916. The results obtained show that we have selected 22 lovastatin hyper producing lines in total 43 mutant strains of A. terreus strain ATCC 20542 and A. terreus VTCC-F916. This is the first step to study the scientific basis for further research on the biosynthesis of lovastatin ability of strains of A. terreus.
Keywords: Aspergillus terreus ATCC 20542, Aspergillus terreus VTCC-F916, lovastatin, 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMG-CoA).
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