Summary

Xanthine Oxidase Inhibitory Activity, Acute and Subchronic (28-day)

Oral Toxicity Study of n-Butanol Fraction of Siegesbeckia orientalis L.

Among 4 fractions prepared from Siegesbeckia orientalis L. (Asteraceae) - a medicinal plant used in Vietnamese traditional medicine to treat gout and related symptom, n-butanolic fraction demonstrated xanthine oxidase (XO) inhibitory activity at 100 mg/ml, showing over 50% inhibition. It had potent enzyme inhibition with an IC₅₀ value of 69.89 (61.19 - 82.54) mg/mL. The effects of acute and subchronic administration of n-butanol fraction of Siegesbeckia orientalis were studied in mice and rats. After oral administration, acute toxicity study suggested a maximum non-lethal dose of 2400 mg/kg. In the subchronic study, rats of both sexes were orally treated with n-butanol fraction ofSiegesbeckia orientalis at 360 mg/kg for 28 days. There were no changes in clinical observations, food and water intake, or urine and feces output. Data analysis of body weight gain, blood biochemical parameters, haematology and histopathological findings did not show significant differences between control and treated groups.

Keywords: Siegesbeckia orientalis; n-butanol fraction; xanthin oxidase; inhibitor; acute toxicity; subchronic toxicity.

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