Vu Hoai Sam*, Duong Thi Phuc Hau, Ta Nhu Thuc Anh,

Nguyen Thi Huong, Pham Thanh Huyen, Nguyen Minh Khoi

National Institute of Medicinal Materials, Vietnam

*Corresponding author: samthavn@yahoo.com

(Received May, 10^th, 2016)

Summary

In vitro Conservation of Dendrobium nobile Lindl. and Anoectochilus setaceus Blume. by Slow Growth Culture

The aim of this work was to develop protocols for slow growth conservation of Dendrobium nobile Lindl. and Anoectochilus setaceus Blume. Shoots of D. nobile were maintained at 25⁰C in a 1/4 MS culture medium containing 5 g/l sucrose and that of A. setaceus in 1/2 MS with 10 mg/l sucrose. After a period of six months without any subculture, shoots of both species were recovered on full strength MS medium containing 30 g/l sucrose plus 6 g/l agar supplemented with a) 1 g/l pepton, 10% coconut water, and 0.5 mg/l Kin for D.nobile and b) 30 % potato extract and 1.5 mg/l Kin for A. setaceus. Retrieved shoots were raised into plantlets and acclimatized in nursery with high survival rates.

Keywords: In vitro conservation, Dendrobium nobile, Anoectochilus setaceus, Slow-growth culture.