\n\tSummary

\n\tStudy on Superoxide Anion Free Radical Scavenging Activity and Effect on Hepatic Glutathione Peroxidase of the Extracts from Ganoderma lucidumand Ganoderma colossum

\n\tEthanol extract, aqueous extract, methanol extract, total polysaccharide and fractionated extracts (diethyl ether, n-butanol, water fractions) from Ganoderma lucidum and Ganoderma colossum were used for in vitro study. Superoxide anion free radical scavenging activity was determined by the 2,4-Iodophenyl-3,4-nitrophenyl-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium method. Superoxide dismutase was used as positive control. For in vivo study, a model of cyclophosphamide-induced liver damage was applied to determine effect of these extracts on glutathione peroxidase (GSH-Px). Silymarin was used as positive control. The results revealed that n-butanol fraction extracted from Ganoderma lucidum and methanol extract from Ganoderma colossum had the strongest superoxide anion scavenging activity. Ethanol extract and aqueous extract from Ganoderma lucidum and Ganoderma colossum[S1] , as well as silymarin, alleviated the increase of hepatic GSH-Px caused by cyclophosphamide. Total polysaccharide extracted from Ganoderma lucidum and Ganoderma colossumhad no significant effect on cyclophosphamide-induced increase of hepatic GSH-Px.

\n\tKeywords: Superoxide anion free radical scavenging activity, glutathione peroxidase, Ganoderma lucidum, Ganoderma colossum.

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