Nguyen Quang Nam*, Tran Hung

University of Medicine and Pharmacy Ho Chi Minh City, Vietnam

*Corresponding author: ginapharm14a@yahoo.com

(Received November, 18^th, 2015)

Summary

Simultaneous Quantitative Determination of Anthraquinones in Aloe vera (L.) Leaves in Vietnam

From Aloe resin, 3 compounds were isolated and determined as aloin A (98.71 % by HPLC-PDA), aloin B (98.67 %) and aloe emodin (99.75 %). An HPLC-PDA method for simultaneous determination of aloin A, aloin B and aloe emodin was developed using a C₁₈ column (5µm, 150×4.5 mm) with linear gradient mobile phase of acetonitrile : formic 0.1 % (20:80 to 45:55) at flow rate of 1ml/min. Detection wavelength of  355 and 254 nm was used for aloin A/B and aloe emodin. The linearity range of aloin A was 2.3 – 1736 µg/ml, aloin B was 1.7 – 1264 µg/ml, and aloe emodin was 0.2 – 64 µg/ml with correlation coefficient of 0.9994, 0.9989, 0.9987 and the regression equation of Y = 15805X - 22135, Y = 15372X + 52821, Y = 76280X - 122993, respectively. The method showed good precision (% RSD: 1.3 – 4.2 %) and recovery (88 – 103 %). The developed method was applied to determine aloin A/B in fresh leaves. In the pulp, it ranged from 0.018 to 0.053 % and from 0.07 to 0.23 (g/g of dry weight) for aloin B and aloin A, respectively. In the rind, the content of aloin A and aloin B were 0.109 – 0.207 % and 0.26 – 0.62 (g/g), respectively. In this work, aloe emodin was not detected in fresh leaves and proved to be an artefact of barbaloin after harvest.

Keywords: Aloe vera, Barbaloin, Isobarbaloin, Aloe emodin, Quantitative determination.